PCR-reverse dot hybridization

  PCR-reverse dot hybγ€ridization is commonly used for genoty≥πping and gene mutation detection. The α&β₩main principle is to point ×γthe probes to be used to the nitrocellulπ≠•ose membrane or the nylon membr®✘ane, each probe is marked wit≤€ε‌h a number,  and↕ ↔  then the DNA sample π §↕to be tested (usually the product specificα☆→ally amplified by PCR) , The biotin label is pr ₩γλe-labeled on the 5 'end of the PCR primer, so th ≤ at the amplified product i‍¥♠αs correspondingly labeled with biotin) ∞♣λto hybridize with it, so that the s↔♦ ample to be tested wi₹× ₩ll be combined with a probe ¥∏★having a homologous sequence,δ✔ and unbound DNA is washed to remoσα✘♥ve In the sample, since the DNA sample to be"♦​£ tested has biotin-based markers, the p×∞robe points combined with the DNA to be t∞★↑σested carry biotin-baσ↑sed markers, and then the hybridization £λ€‍signal can be displayed by the corresponding‌φ color reaction.