The main principle of PCR fluore←™scent probe melting curve technology is to Ω✘✔ design molecular beacon probes Ω₩for the sites to be detecγ↔★ ted; after PCR amplification is compl>÷☆αeted, the amplified produ∑ cts are hybridized with mol♥'✘ecular beacon probes, then slowly rise Te↓≠mperature, do the meltingγ∑ curve analysis of thα₩e probe and the product, and read¥γ the genetic polymorphis≠∑m information of the site according to the t'πemperature corresponding to the melting p↔♥eak. Compared with other methods, it has tλ≤he advantages of simple operation,±< high accuracy, good specificity> and easy to judge results.
Date:2020-03-26
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