PCR-electrophoresis

  The main principle of the PCR-↓♦☆electrophoresis method (Ga ​p-PCR method) is to conduct electropho↑↔resis of PCR products; and₩↑ the charge effect and molecular sieve eff₹♦ect of DNA molecules while swimming. Under π γa certain electric field strength, the migration  ∑speed of DNA molecules ✘∞•depends on the molecul¶®ar sieve effect, that is, the size and configurat®≠<ion of the molecules themselves. Therefore↓€≥, different DNA molecules a¶≤‌re isolated and compared with Marker to determi Ωπ•ne the result. The PC>↕R-reverse point hybridization methoδ®>♣d uses a biotin-labeled p↓¶βrobe to hybridize with a specific amplified PC£✔≥R product, and a hybri≥♥§dization signal can be displayed through a corre←↓sponding color reaction to judge ₹¥☆δthe result.