HiPure Viral Nucleic Aicd Isolation Kit

BACKGROUND

Molecular diagnosis and ÷ nucleic acid detection have≠₩ been widely used in thediagnosis of infectiousγ or genetic diseases,£≤ non-invasive prenatal detection,e¥ππ∞pidemiological investigation, pathogenic microor<≈φganisms detection and sφ♣cientificresearch. Comp& ared with immunological detect∑£ ↑ion techniques, nucleic a‍✘₩cid detectioncan obtain more sensitive and acc✔©urate results. The first step♥> ↓ in molecular diagnosi δ‌£sinvolves the extraction of ‌→nucleic acids, which ar><e usually bound to organic matter ince¥®•→lls, such as histones. The extraction of nucleiε↑←δc acids requires first lysi≈‍ng the cells,and then seπ∏parating the nucleic acids f¥→rom macromolecular organic substances (suchas pr✘∑oteins, polysaccharides, and lipids), while the p‍φrimary structure of ≈±$✔the nucleicacids must remain intact. The nuclβ₩<eic acid extraction process currently≥™ depends onqualified profess<≠<ionals to operate. However,γ≈∞ with the rapid development of nucleicacid☆β detection technologies and cl≈£εγinical diagnosis, there is an u'♣‍rgent need for asimple and✔✘ easy-to-use method su÷₽™←itable for on-site DNA extraction and detection.Tλ→∏his method needs to be ✔∑β♠widely applicable to technical requirements foα≥ r nucleic acidextraction from pδε≈"rimary hospitals, testing &∏Ω₽sites and even the general public.

BASIC PRINCIPLES

This product is based on silica gel®¥βα column purification. T§he sample is lysed, and theDNA/RNA ₹∑is released into the lysis b≠•≥uffer. After transferring to an adso→£rption columnand filter, DNA/RNA is adsorbed ★∏×to the membrane, while the unabsorbed ∏©‌ protein isremoved by filtration with the solutioδ<n. After washing away the protein and otherimpuri™ ↓≠ties, DNA/RNA is finally el↔↑uted by a low-salt buffer to obtain©↕✔ a high-purityproduct

PRODOUCT INFORMATION

Applicable specimens: ‍φσcell-free/low-cell DNA/∞¶&RNA samples such as clinical or humanbody fluids'∏, serum, plasma, soakin÷ αg fluid, supernatants from tissue♣✔∑ homogenate, cellculture su♠♠✘∑pernatant.

Technical principle: silica gel column purifΩγication.

Packing specification: 9β≠6 tests/kit, 200 tests/kit.

Storage conditions: 18-25℃ room temperature for 12 month∏"s.

PRODOUCT PERFORMANCES

HIGH RECOVERY RATE

silica gel column can recover nucλ‌leic acid moleculesα→∑ at the picogram level.

HIGH SENSITIVITY

Recovering viruses DNA/RNA as low as ± 10 copies.

HIGH QUALITY

Meeting various downstream applications ©‍>such as reverse transcription,qPCR,enzyme digesti ÷₩ on,blot hybridization✘≥✔,etc.

GOOD STABILITY

Stable solution system ensures conσαsistent results every tim∑∏∞εe.