
Molecular diagnosis and nuγ©≥®cleic acid detection have ♣<been widely used in thediagnosis of infectious or¥☆∏λ genetic diseases, non-✘©©•invasive prenatal detecti₽$on,epidemiological investigation, patho§δ☆genic microorganisms detection and sc♦↕ ♣ientificresearch. Compared with i₩♠mmunological detection techn♦¶€iques, nucleic acid detectioncan o¥÷® btain more sensitive and accurate results. ¶πThe first step in molecular diagnosisinvo ÷↕<lves the extraction of nu₽©cleic acids, which are usually₹σ bound to organic matter incells, such a≠§πs histones. The extraction of nucleic✔' acids requires first lysing the cells,and th•↔↓αen separating the nucleic acids from macromoσ₽₹lecular organic substances Ω♠(suchas proteins, polysaccharidδ ™βes, and lipids), while the primary structure of ±∞the nucleicacids must remai↔↑φn intact. The nucleic acid extraction ε≤¥process currently depends onqualifie∏γ≤d professionals to operate. However, with th∑★e rapid development of nucleicacid detectio✘≥ n technologies and clinical σβdiagnosis, there is an urgent need for asimple an> d easy-to-use method sui'÷δtable for on-site DNA extraction and detec¶÷•tion.This method needs to be widely applicΩ≠¶≤able to technical requirements for nu♣"≥δcleic acidextraction from primary hospitalσ÷£s, testing sites and even the general pub↔λ$lic.
This product i"'s based on silica gel co€£§→lumn purification. The sample ♣$®©is lysed, and theDNA/RNA is ↕₽λαreleased into the lysis buffer. After t₹βransferring to an adsorption c₩×→≤olumnand filter, DNA/RNA isΩ≥ adsorbed to the membrane, while the unabsor☆λ±₹bed protein isremoved by fi®∑ltration with the solution. After washing ∞α<away the protein and otherimpφ♣urities, DNA/RNA is finally elut✔₽₹≤ed by a low-salt buffer to obtain a high-puritypr♦σ≥✔oduct
Applicable specimens: cell>★♥-free/low-cell DNA/RNA samples ±•σsuch as clinical or humanbody fluids, serum, plas α±ma, soaking fluid, supernatants from tissue hoσ★™mogenate, cellculture≤₩★₽ supernatant.
Technical principle: sili<✘ca gel column purific±×γation.
Packing specification: 96 tests/kit, 200 test♦←"s/kit.
Storage conditions: 18-25℃ room temperature for 12 montβ♠hs.
HIGH RECOVERY RATE
silica gel column can recover nucl♥☆eic acid molecules atλ©Ω the picogram level.
HIGH SENSITIVITY
Recovering viruses ∑♠DNA/RNA as low as 10 copies.
HIGH QUALITY
Meeting various downstream appli'★↑§cations such as reverse tran¥☆φscription,qPCR,enzyme digestion,bl βδot hybridization,etc.
GOOD STABILITY
Stable solution system ensures consistent resuεαlts every time.
WIDE APPLICABILITY
Suitable for extraαλ∞cting viral DNA from se$εrum, plasma, milk, cell culture supernatant,and ←×σvarious cell-free body fluid samples.
FAST
Extraction of multiple samples can£γ" be completed in less than ☆λ30 minutes.
Date:2020-03-26
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