L. pneumophila nucleic acid detection kit (PCR-fluorescent probe method)
L. pneumophila nucleic acid detection kit (PCR-fluorescent probe method)

L. pneumophila nucleic acid d€♦etection kit (PCR-fluorescent probe method)β¶

【Clinical manifestations】
  Approximately 90% of Legionellosis↔≥ ÷ is caused by Legionella pneu♠♣mophila. The causative agent of Legi"£•♥onella pneumonia is Legionella pneumophila, wh∏<>ich accounts for 2 to →∑↑☆5% of community-acquired pneumonia . The±π symptoms of Legionella disease vary wi®≤÷≠dely, from common cold and ×♣"low fever to high fever, with changes inσ‍  mental status and respiratory failure; atyp<✘ical symptoms usually occur early in the ™‍disease, including headache, muscle ac>♥hes, anorexia, fatigue, etc. . Diarrhe→ ≤a and other gastrointest✘♣φβinal symptoms are approximately 20π™"-40% . Among the atypical cases of community-acqu₽π‌ired pneumonia, Legionella dise≥"ase is the most serious clinically,≈α and if not treated properly and in time, it ₽×​♣will progressively worsen. Although there are fφ••ew extrapulmonary symptoms, it can also cause ‌♥₽myocarditis, pericarditis and artif★&icial valve endocardi ↑tis, as well as glomerulone ₽'¶phritis, pancreatitis and peritonitis.
【Features】
  This kit uses real-time f®♠luorescent PCR technology, ta₹φkes the highly conserved r¶  ★egion of the Legionella pneumophila genome as t§™he target region, designs specific •≠primers and fluorescent probes, and the↔λ n performs PCR amplification on the t≠​↕ arget sequence , while   >monitoring real-time ex✔"λpansion through Taqman fluores>₩cent probe technology. Accumu™‍♣→lation of augmentation products enables q♠≥∞​ualitative detection of Norovirus nλ©γ÷ucleic acids in samples . The reac™×tion system of this kit contains dU-UNG ©♦>₩enzyme anti-pollution s$±↑₹ystem to avoid false positive results .
【Product Performance】

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

The detection result coincidence rate i♣>s 100%

Precision

Coefficient of varia✘'tion within and between ba♣∑tches CV≤5%

Specificity

100% specificity , no cross™↑ reaction with Staphylo♣÷≥¶coccus aureus, 

respiratory syncytial virus, Epstein☆<‍€-Barr virus, Haemophilus influe$¶↓nzae, 

Mycoplasma pneumoniae, Streptococcus pneumo®♦δniae, etc.

Anti-interference

Oxymetazoline hydrochloride sp•±‍ray, budesonide nasal spray, ery™≠↔thromycin, 

roxithromycin, azithromycin, etc. β ≠did not significantly interfere>♠Ω∑ with the test results

【Product information】
Test specimen: throat swab
Technical principle: PCR- fluorescent ¥>&♦probe method
Packaging specificatiπ☆£ons: 24 tests/ kit, 48 tests/ kit,←ε  96 tests/ kit
Applicable instruments: Roche Cobas z 480 ↔§; Roche LightCycler 480 ; ABI 7500 ; Shan™γ<ghai Hongshi SLAN-96S ; Bioer LineGene 96¶λ€00, etc.