Boca virus nucleic acid detection kit (PCR<♠∏↔-fluorescent probe method)
【Clinical manifestatioπ∞ns】
Bocavirus is a genus of the Parvo¶β¶φviridae subfamily of the Parπ♣✘voviridae, first discovered in a↓±nimals in the early 1960s. In Aδ ugust 2005, Swedish s©σ≠∞cientists used molecular virus ↑αscreening methods for the f♣↔δirst time to discover a new type o"<♦×f human parvovirus in children's respiratoε★→ry secretions, named human Boca virus, which ₹β♣ is closely related t↕↑o human acute respiratory infections, a →÷nd the symptoms are commo©γ ×n Colds are similar. The same viru♣≥s was found in Australia and China, and the€★ infection rate was greater than 5.6%.
【Features】
This kit uses real-time fluoresce ♥←nt PCR technology, takes the highly co®φ≈σnserved region of the Boca virus ♥× genome as the target•€< region, designs specific primers and fluoresα∞cent probes, and then performs €×₹PCR amplification on the target sequence, whil€≥e monitoring the amplif→σ≈ication in real time by Taqman fluorescent <÷probe technology The accumulation of products≤$ enables qualitative detection of Norovirus ↕nucleic acids in the sample. The∏₩≥ reaction system of this kit contain"∏γs dU-UNG enzyme anti-pollution syst×∞em to avoid false positive results×€∏>.
【Product Performance】
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Sensitivity
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1.0x103 copies / mL
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Linear range
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1.0x103 copies / mL ~ 1.> ↕0x 109 copies / mL
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Accuracy
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The detection result coincidence rateδ☆" is 100%
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Precision
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Coefficient of variaε←tion within and between batches CV≤5%
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Specificity
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100% specificity, no cross reaction ←¶×with influenza A virus, influenza B ↕$<virus, adenovirus , hΩδ©uman metapneumovirus, rhinovirus,&n$γbsp;Epstein-Barr virus, Mycoplasma pneumo★ ≤niae, Chlamydia pneumoniae, etc.
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Anti-interference
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Oxymetazoline hydrochloride '¥ sprays, budesonide Ω÷nasal spray, azithromycin ne♥γomycin, zanamivir, ribavirin and the like without sig nificant interference to the detection result
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【Product information】
Test specimen: throat swab
Technical principle: PCR- fluorescent prob±δe method
Packaging specifications: 24 tests/ kit, 48 test↕←®'s/ kit, 96 tests/ kit
Applicable instruments: Roch®φe Cobas z 480; Roche LightCycler₩✘ 480; ABI 7500; Shanghai Hongshi SLAN-96S;÷λΩ≠ Bioer LineGene 9600, etc.
