Norovirus nucleic acid detection kit (PCR-fluorescent probe method)
Norovirus nucleic acid detection kit (PCR-fluorescent probe method)

Norovirus nucleic acid detection kit (PCR-fluor¥β↓βescent probe method)

【Clinical manifestations】

  Norovirus  is considered t☆β£o be the major cause of no•>n-bacterial pathogen ou‌±tbreaks of acute gastroenteriti₹↔↔s, often causing vomiting, diarr​♥φhea and other symptoms, can be transmitt₹♣↑ed through a variety of wayδ•'×s food, water and so on. Norovirus virus dividφ‌"¥ed . 5 genome, wherein ​γβG Ⅰ, G Ⅱ Norovirus is the most common worldwide ∞ ♥genome, reported to h‌∏♠↑ave a global epidemic diffusion. Noroviru®♥s is mainly infected through♠™£ the fecal-oral route. After infection, humans an♥≤d animals can secrete a large number of nor₹←'ovirus particles. Innovirus has stα®♥↓rong viability and infectivity in vitro. Noroviru×​✔s can be infected in small doses (<100 ≈≤ ®virus particles), and the population i''s generally susceptible to it. The clini§£γcal symptoms of the human body after being>₩± infected with Norovirus are mainly vomi ©§ting and diarrhea, and it is difficult to disti​÷♥nguish the symptoms of gastroenteritis caused↔ →® by other pathogens.

【Features】
  This kit utilizes rea☆ ≥&l-time fluorescence PCR technology to norovi∑γrus G Ⅰ, G Ⅱ type genomic r∏₹>φegion is highly conserved↓₽₹ target region, the design of¥β© specific primers and fluoφ✘δ→rescent probes, and εγ≈≠target sequences for PCR amplification, throu‌Ωgh Taqman fluorescent probes N<∞₩≠eedle technology monitors the accumulation of am" ×‌plification products i¶∑≠±n real time, enabling qualitative detection±↔ of Norovirus nucleic acids in samples . The reac✔↑→tion system of this kit contβ≤×ains dU-UNG enzyme anti♥↑€-pollution system to avoγγ&φid false positive results .
【Product Performance】

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

The detection result coincidence rate is 100%

Precision

Coefficient of variation within and betw✔↓≈¥een batches CV≤5%

Specificity

A specificity of 100% , w♥≥ ith the wheel-like virus, Sap>₹₽φporo virus, 

enteric adenovirus virus, stellateεπ virus, Salmonella, 

Shigella and other non-cross-rea©&♠'ctive

Anti-interference

10% pus blood, 0.5% erythromycin suppository,&nb☆Ω'sp;

0.5% anal Thai suppository, etc. d§∞‍φid not significantly ↓↕₩πinterfere with the t₩☆≥&est results

【product information】
Test specimens: feces, anal swabs
Technical principle: PCR- fluorescent pro≈↕be method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 480 ; Roche☆€ LightCycler 480 ; ABI 7500 ; Shanghai H←≠ongshi SLAN-96S ; Bioer LineGene 9600, etc.