Shenzhen Yilifang biotechnology Co., ltd.
Detection of genetic diseases, detection of infectious diseases of the respiratory tract, detection of tumor early or prognosis, detection of karyotype analysis

SARS-CoV-2 Nucleic Acid detection Kit(Real-time Pλ∞CR)

【Background】

     In 2020, an une>≈xpected outbreak swept the world. The main pa÷↓thogen causing the outbreak is SARS-≤ CoV-2, a new virus that r÷☆esearchers first identifiedλ±  in Wuhan province, China, in late 2‌®019, and was named by the World Health Orgaγ≈‍✘nization on January 12, 2020. It is a♥€₽ε pathogen that mainly causes 'δ★£respiratory and intestinal diseβ↓ases.
The epidemic coincided with the&  Spring Festival in 2020, and the developm≠♣ent was very fierce and₩'≠γ spread widely. As an in ☆dustrial national high-tech&> enterprise for the det✔β↕$ection and development of‍₩¶Ω respiratory pathogens, our co​↓σmpany joined the "battle" as soon as possible a₹​<nd quickly organized the strongest R & D for←<δce to succeed Develop ®≠high-sensitivity detection kits€☆™¥ to fight against vir¶δ÷uses and help the frontline fight ★→against epidemics!

【Clinical Manifestations】

Based on the curr©‌βent epidemiological survey, the incuba®★​®tion period for 2019-nCoV is generally™≤• 3-7 days, with a maximum of 14 days. The clini✘>≤§cal manifestations of the vi←¥→πrus infection are: fever, fatigue, rα♥↓<espiratory symptoms a&•αre mainly dry cough, and dyspπ ©nea gradually develops; severe cases present wit∏↑♠‌h acute respiratory dγ€istress syndrome, septic s​$α₹hock, metabolic acid∞♠∑§osis and coagulation"☆¶ that are difficult to treat; some patie∞≠nts have mild onset symptoms but no feve φ☆δr; most patients have a good prognosis, and a few​✔≤  patients are in critical co•€α÷ndition or even die. The dise∏​ase caused by the virus can π↔"be manifested as simple infection, m§£ild pneumonia, severe pφ≈neumonia, ARDS, sepsis, and septic shock. Accor≠λding to the severity of clinical syndr®‍ome, it is divided i✘↕nto mild, moderate and severe, the latter←÷ β including severe pneumoni×↑α>a, ARDS, sepsis and septic₩φ™≈ shock.

【Product Features】

Based on real-time fluorescent  ‌PCR technology, the resultα$÷♦s are produced within 1.5 ★♥¥ hours.

The reaction system of this k®₽♣☆it contains a dU-UNG €±enzyme anti-pollution system to avo→​§∞id false positive results; the system  ₹↓‌contains internal standards<★× to avoid false negative results ™‍.

Taking the highly conserved regions of t×₹♥he ORF1ab and N genes i‍✘♥n the genome of the new coronavirus (2019-ε↕nCoV) as target regions, ↔σ'εspecific primers and fluorescent probes were de®×signed to perform PCR amplification >≠←to ensure the accuracy of the product.

【Product performances】 ∑

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

100% in enterprise references

Precision

Coefficient of variation (→βCV) ≤5% within and between ba₩↓€tches

Specificity

No cross-reaction with coronavirus ('↕NL63, HKU1, 229E, OC43), influenza A virus, influ≠✘ εenza B virus, respiratory syn≤¶cytial virus, adenovirus, mycop✔‌lasma pneumonia, and bordetella pertussis.™™

Interference

Not affect by Interferon  ↕ γ(α-2b≤180 μg/mL), Hydrox≈αymezoline hydrochloride spray↑∑(≤150 μg /mL), Budesonide nasal÷♣≤> spray(≤256 g /mL), Erythromycin(≠  ↔≤50 mg/mL), Roxithromycin (≤7.5¥∞& mg/mL), Azithromycin (≤100mg/mL).

【Product Information】
Applicable Samples: pharyngαε• eal swab, sputum, alveo≈Ωlar lavage fluid

Technical Principle:Realγ∑-time PCR

Packing: 24 tests/kit, 48 tests/ki>←t, 96 tests/kit

Applicable instruments:ABI 75≈♥00,ABI 7300,Roche480,Roche96, etc£↑☆.