Taqman one-step real-time fluorescence RT-PCR detection kit
Taqman one-step real-time fluorescence RT-PCR detection kit

Taqman one-step real-time fluoresc↔€<ence RT-PCR detection kit

Product Brief

   Taqman one-step real-♥♦₹€time fluorescence RT-P←÷♣CR reagents include 25 × φΩ↑RT-PCR mixed enzymes, 2 × OneStep RT-P±₽≈CR Buffer and RNA / DN®∑♣ase-free water. It is a special ₹♥★→reagent for Real Time One Step RT-PCR by hydro​"lysis probe method After adding the tem<γ•plate and primer probes, onα♥₽e-step method can achieve high effi↑λ"γciency of reverse transcription and P♦±→CR amplification in one tube. It is also suita±☆ ble for Roche PCR amplifier∑¥$s that use glass capillaries and dλ♥ oes not require the addition of BSA.  ​≤↑This product can also be±β used for rapid Real Time ★•<PCR amplification reactions. Thφ×is product can get a good standard curve in←π☆♥ a wide quantitative area, accurate quantitative↑® detection of targetφπ genes, good repeatability and high reliabili★ ty.

Product advantages
1. There is no need to open the lid during the RN​♦ >A amplification process, so there is le₹$☆€ss pollution.
2. Reagents are spare type, ju​♥st add template and primer probe, easy to oper‍∑ate.
3. There is no preference in the re₩→verse transcription process. S'&™™imultaneous reverse transcriptio÷>α←n of high-abundance and low-abundance€ δ$ RNA in the same tube can¥≈→∑ improve the sensitivity ☆×and accuracy of the detection  ∏ and the repeatability is good.
4. Strong applicability, whether it is a↑£ λ high GC ratio template or RNA with a complicat✔§∑₹ed secondary structure, can e♣¥γasily perform reverse traα​Ω•nscription and PCR.
5. A good standard curve can be obtained in a ♦ wide quantitative area, and experimenσ≈<♦tal verification can obta←₹®in a standard curve of at least≥↑÷♦ 9 orders of magnitude, and even a small amo÷≠♦δunt of template can ✔€be accurately quantitatively detected.
6. The reagent contains Rox fluorescence≥' correction, eliminates the differenc ★$"e between the detected wells, ​≈and can be used to standardize the quantit©≈★ative fluorescence signal




The Taqman one-step real-t≤>±δime fluorescent RT-PCR detection ki λ•t is used for quantitati£ε≠ve quantitative analysis of the target gene fπ✘or accurate quantitative dete¶☆×£ction, with good repeatab ₽♠σility and high reliability.



Applicable instruments

Fluorescence quantitative PCR instru←≈↓σment