Adenovirus nucleic acid detection kit (PCR-fluorescent probe method)
Adenovirus nucleic acid detection kit (PCR-fluorescent probe method)

Adenovirus nucleic acid"∞ detection kit (PCR-fluorescent ≈Ωφ↑probe method)

Clinical background

  Adenovirus (ADV♣♦ ¥) is a group of very w≠' idely distributed viruses wiβγ≤th double helix DNA genomes. Acute infectious d≈÷$→iseases caused by AD¶≥®×V can easily invade  ∑the mucous membranes of the resp$±€iratory tract and digestive tract, ocular con←≈≤£junctiva, urinary traλ♣ct and lymph nodes, mainly manifested as♥☆ acute upper respirator↔™y tract. Infection (2% to 4% of ac♥φ↔ ute respiratory infections caused by ad×σenovirus), followed by eye and gastroin¶₽$•testinal infections. T✘♣he population is generally susceptible, more♥♣ common in children, about hal₽$"f of the patients are h±δidden infections. Infants an÷¥<<d young children are susceptib ☆ ¥le to adenovirus pneumonia, which accounts for a‍σλΩbout 10% of childhood pβ$neumonia. The condition is <÷©σserious and the mortality is ¥↕high. The mortality of ade✔∏novirus pneumonia in young people is 8% to 10>♦≥'%, and there is no specific treatment.δ→ According to the investigation §≈​ of serum specific an>♥tibody and virus isolation, it can be€∞☆ seen that adenovirus infection is v♣∏≥ery extensive.

Precision and linear range


Figure 1: Precision (using 1.0x104PFU / mL ref♦♣σ®erence material as a template, repeated 2β★ 0 times in the same batch to det$↕ect CV value ≤5%)
Figure 2: Linear range (for 103 ~ 108PFU / mL con↕<centration range refe♦¥≈≠rence products, the pr≥↓δ oduct amplification curve is smootΩ±≈h and complete, the correlati✘≠₩on coefficient between diff​☆≈→erent gradients is R> 99%, and the correla♥≠Ω©tion is good)



Product performance

Adenovirus nucleic acid detection kit (PC×€>R-fluorescent probe method)

Sensitivity

1.0x103PFU/mL

Linear range

1.0x103PFU/mL ~ 1.0x≤×​€ 108PFU/mL

Accuracy

The positive coincidence rate was 100%, the ↓↑‌negative coincidence r♦÷§ate was 98.83%, and the overall coincidence ​£₽αrate was 99.41%

Precision

Coefficient of variation within and between b'φλ‍atches CV≤5%

Specificity

100% specificity, no cross ♥¶reaction with other respiratory patho‌π>gens

Anti-interference

Mucin, blood, pus, erythromycin∑✘¥, chloramphenicol did not interfere w‌₩★ith the test results

product information
Test specimen: throat swab
Technical principle: PCR-fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 testδ₹×εs/ kit
Category: In vitro diagnostic ∞₽reagents
Applicable instruments: fluorescence quantitative PCR≤×‌‌ instrument